Khanna, Pyare; Snyder, Evan S.; Singh, Prithipal;

Chloramphenicol derivatives antigens and antibodies

Chloramphenicol derivatives are provided for use in the preparing of antigen conjugates for the production of antibodies specifically for chloramphenicol. Specifically, the aryl amino group is derivatized to introduce a non-oxo-carbonyl group which is used for amide formation with an antigen. The conjugate is then injected into a vertebrate for production of antisera which is isolated in conventional ways and find particular use in competitive protein binding assays.

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What is claimed is:

1. Antibodies prepared in response to an antigen of the formula: ##STR4## wherein: R is a saturated aliphatic linking group of from 2 to 6 carbon atoms having from 0 to 1 oxo-carboxyl groups at its terminus bonded to nitrogen;

Y.sup.1 is a poly(amino acid); and

n.sup.1 is one to the molecular weight of Y.sup.1 divided by 2000.