Hodgson, John Edward; Jenkins, Owen; Sarginson, Gillian;

FtsH from Staphylococcus aureus

The invention provides ftsH polypeptides, polynucleotides encoding ftsH polypeptides and related polynucleotides and methods for producing such polypeptides by recombinant techniques. Also provided are diagnostic methods for detecting Staphylococcus aureus.

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What is claimed is:

1. An isolated polynucleotide segment comprising nucleic acid sequence comprising nucleotides 1 to 2091 of the polynucleotide sequence set forth in SEQ ID NO:1, or the full complement of the entire length of the nucleic acid sequence; wherein the nucleic acid sequence or the full complement thereof is not genomic DNA.

2. An isolated polynucleotide segment comprising a first polynucleotide sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2; wherein the first polynucleotide sequence is not genomic DNA.

3. A vector comprising the isolated polynucleotide segment of claim 2.

4. An isolated host comprising the vector of claim 3.

5. A process for producing a polypeptide comprising culturing the host cell of claim 4 under conditions sufficient for the production of said polypeptide wherein the polypeptide comprises SEQ ID NO:2.

6. An isolated polynucleotide segment, comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence hybridizes to the full complement of SEQ ID NO:1, wherein the hybridization conditions include incubation at 42.degree. C. in a solution comprising: 50% formamide, 5.times.SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5.times.Denhard's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1.times.SSC at 65.degree. C.; wherein the first polynucleotide sequence is at least 95% identical to SEQ ID NO:1; wherein the first polynucleotide sequence or the full complement thereof is not genomic DNA and wherein the first polynucleotide sequence or the full complement thereof detects Staphylococcus aureus by hybridization.

7. A vector comprising the isolated polynucleotide segment of claim 6.

8. An isolated host cell comprising the vector of claim 7.

9. An isolated polynucleotide segment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO:2; and wherein the first polynucleotide sequence or the full complement thereof is not genomic DNA.

10. A vector comprising the isolated polynucleotide segment of claim 9.

11. An isolated host cell comprising the vector of claim 10.

12. A process for producing a polypeptide comprising culturing the host cell of claim 11 under conditions sufficient for the production of said polypeptide, wherein the polypeptide consists of SEQ ID NO:2.

13. The isolated polynucleotide segment of claim 6, wherein the first polynucleotide sequence is at least 97% identical to SEQ ID NO:1.

14. A vector comprising the isolated polynucleotide segment of claim 13.

15. An isolated host cell comprising the vector of claim 14.

16. An isolated polynucleotide segment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence is at least 95% identical to SEQ ID NO:1, and wherein the first polynucleotide sequence or the full complement thereof is not genomic DNA; and wherein the first polynucleotide sequence or the full complement thereof detects Streptococcus pneumoniae by hybridization.

17. A vector comprising the isolated polynucleotide segment of claim 16.

18. An isolated host cell comprising the vector of claim 17.

19. The isolated polynucleotide segment of claim 16, wherein the first polynucleotide sequence is at least 97% identical to SEQ ID NO:1.

20. A vector comprising the isolated polynucleotide segment of claim 19.

21. An isolated host cell comprising the vector of claim 20.

22. The isolated polynucleotide segment of claim 16, wherein the first polynucleotide sequence is at least 98% identical to SEQ ID NO:1.

23. A vector comprising the isolated polynucleotide segment of claim 22.

24. An isolated host cell comprising the vector of claim 23.

25. The isolated polynucleotide segment of claim 16, wherein the first polynucleotide sequence is at least 99% identical to SEQ ID NO:1.

26. A vector comprising the isolated polynucleotide segment of claim 25.

27. An isolated host cell comprising the vector of claim 26.