Sutcliffe, J. Gregor; Erlander, Mark G.; Lovenberg, Timothy W.;

DNA encoding serotonin receptors

The present invention describes nucleic acid molecules encoding human serotonin receptors, recombinant serotonin receptor proteins, cultured cells expressing recombinant serotonin receptor proteins, antibodies immunoreactive with serotonin receptor proteins, polypeptide serotonin receptor antagonists, oligonucleotide probes used for detecting nucleic acids which encode a human serotonin receptor, and nonhuman transgenic animals which express recombinant human serotonin receptor. Also disclosed are methods for screening for ligand binding to the described serotonin receptors and for serotonin receptor agonists and antagonists, for detection of serotonin receptors in tissues, and for therapeutic treatments involving the described human serotonin receptors.

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What is claimed is:

1. An isolated DNA segment encoding a 5-HT.sub.5.alpha. serotonin receptor REC17 comprising a nucleotide sequence that codes the amino acid residue sequence shown in SEQ ID NO 4.

2. The isolated DNA segment of claim 1 wherein said DNA segment comprises the nucleotide sequence shown in SEQ ID NO 3 from base 150 to 1220.

3. A vector comprising the DNA segment of claim 1, wherein said vector is adapted for expression of said serotonin receptor in a host cell containing said vector.

4. The vector of claim 3 wherein said vector is adapted for expression of said serotonin receptor in a mammalian host cell.

5. An isolated mammalian cell comprising the DNA segment of claim 1.

6. The isolated mammalian cell of claim 5 wherein said DNA is present in a vector adapted for expression of said serotonin receptor in said mammalian cell.

7. The isolated mammalian cell of claim 6 that further comprises a cell surface serotonin receptor expressed from said vector.

8. An isolated DNA segment encoding a 5-HT.sub.5.beta., serotonin receptor MR22 comprising a nucleotide sequence that codes the amino acid residue sequence shown in SEQ ID NO 2.

9. The isolated DNA segment of claim 8, wherein said DNA segment comprises the nucleotide sequence shown in SEQ ID NO 1 from base 303 to 1412.

10. A vector comprising the DNA segment of claim 8, wherein said vector is adapted for expression of said serotonin receptor in a host cell containing said vector.

11. The vector of claim 10 wherein said vector is adapted for expression of said serotonin receptor in a mammalian host cell.

12. An isolated mammalian cell comprising the DNA segment of claim 8.

13. The isolated mammalian cell of claim 12 wherein said DNA is present in a vector adapted for expression of said serotonin receptor in said mammalian cell.

14. The isolated mammalian cell of claim 13 that further comprises a cell surface serotonin receptor expressed from said vector.